Azura 1-Step Ultra RT-PCR Kit
Azura 1-Step Ultra RT-PCR Kit has been formulated for cDNA synthesis and subsequent PCR in a single tube for end-point analysis. This new generation RT-PCR Kit consists of a thermostable reverse transcriptase, a potent RNase Inhibitor and Azura HS Taq for ultra-sensitive 1-step RT-PCR from as little as 1pg total RNA starting material.
The kit also efficiently synthesizes double-stranded cDNA for subsequent gene expression analysis.
In order to confirm the ideal reagent for your application, please click here for our PCR Selection Guide.
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The advanced and highly optimized buffer chemistry allows for efficient reverse transcriptase and PCR of problematic sequences with significant secondary structure (GC-rich targets).
The Azura 1-Step Ultra RT-PCR Kit is ideal for determining the presence or absence of RNA templates and quantifying expression through qualitative analysis of RNA transcription levels.
Applications
- Gene Expression analysis
- Multiplex RT-PCR
- Transcription analysis
Sensitivity
Optimized chemistry for detection of low-copy number transcripts
Robust
Overcomes secondary structure in problematic GC-rich targets
Convenience
First strand full-length cDNA synthesis and PCR in a single tube from a wide input range of total RNA
Manual:
Azura 1-Step Ultra RT-PCR Kit Manual
Product Sheet:
Azura 1-Step Ultra RT-PCR Kit Product Sheet
Safety Data Sheet: