PureView™ Prestained Protein Ladder
- Ready-to-use three-color protein standard with 13 pre-stained proteins
- covers a wide range of molecular weights from 5 to 245 kDa in Tris-Glycine buffer (3.5 kDa to 235 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer)
- Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa, respectively) when separated on SDS-PAGE (Tris-Glycine buffer)
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The PureView Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (nitrocellulose, PVDF, or nylon) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use.
Do NOT heat, dilute, or add reducing agents before loading.
Loading Volume:
3 µl or 5 µl per loading for clear visualization during electrophoresis on a 15-well or 10-well mini-gel.
1.5~2.5 µl per well for general Western blotting transfer.
Apply more for a thicker (> 1.5 mm) or larger gel
Contents
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).
Quality Control
Under suggested conditions, PureView Prestained Protein Ladder resolves 13 major bands in SDS-PAGE (Tris-Glycine, MOPS, and MES buffer) and after Western blotting transfer to a nitrocellulose membrane.
Storage
4°C for 3 months
-20°C for 24 months