AzuraQuant™ II cDNA Synthesis Kit

The AzuraQuant™ II cDNA Synthesis Kit is supplied with a high-performance 5x cDNA synthesis mix including an optimized ratio of random hexamer primers and anchored oligo (dT) primers, which delivers excellent consistency. The AzuraQuant™ II cDNA Synthesis Kit delivers both highly efficient first-strand synthesis and higher cDNA yields, leading to enhanced reproducibility and data accuracy. The kit is supplied in a convenient 2-tube format comprised of a 5x cDNA synthesis mix and 20x HighTherm™ II reverse transcriptase blended with RNase inhibitor. HighTherm™ II reverse transcriptase exhibits enhanced thermostability and processivity, low RNase H activity, and robust overall performance in cDNA synthesis.

Applications

• 2-step RT-qPCR
• 2-step RT-PCR

Convenience
Two-tube format and simple 25 minute protocol
Unbiased, Sensitive cDNA Synthesis
Complete 5' to 3' RNA sequence representation from 4 pg to 2µg Total RNA

Reproducible
HighTherm™ II reverse transcriptase coupled with an optimized 5x cDNA Buffer system for consistent, high-yield qPCR-ready cDNA.

Broad dynamic range
High-yield cDNA synthesis across a wide range of RNA concentrations.