AzuraFlex™ cDNA Synthesis Kit

The AzuraFlex™ cDNA Synthesis Kit is a complete and flexible system for efficient synthesis of first strand cDNA from Total RNA or mRNA templates. The MMLV-derived reverse transcriptase (High Capacity RTase) is modified for improved sensitivity with challenging templates, and greater thermostability. The enzyme, which is capable of synthesizing cDNA up to 9 Kb is blended with RNase inhibitor for greater stability and performance with a wide range of RNA sources.

• Includes separate solutions of random hexamer primers and oligo (dT) primers for assay flexibility.
• System generates consistent, high-yield qPCR-ready cDNA fragments up to 9Kb in length.
• Optimized for a wide range of input RNA of 10 pg to 2 µg Total RNA.
• Downstream applications include real-time PCR, standard PCR, and microarrays.
• Optimized 5x RTase Buffer Mix includes optimal levels of dNTPs and MgCl2 for reduced pipetting steps.