PureView™ 100 bp DNA Ladder

Azura PureView™ DNA Ladders are a complete range of cost-effective, ready-to-load molecular weight markers including loading buffer in a single tube, enabling simple and accurate DNA sizing. For easy visual identification and orientation, PureView™ DNA Ladders also contain high-intensity reference bands. The ready-to-load format minimizes the time spent thawing, diluting and adding tracking dye to the DNA ladder. Simply transfer 5 µl of PureView™ DNA Ladder from the vial to the gel.

Concentration

0.1 mg/mL

Storage Conditions

PureView™ 100 bp DNA Ladder is shipped at 4°C. On arrival, store at -20°C for 36 months, 4°C for 12 months or 25°C for up to 6 months.

Quality Control

PureView™ 100 bp DNA Ladder is extensively tested prior to release using a 2% agarose gel to verify the sharpness of each band and a spectrophotometer for DNA concentration.

Protocol for Use

Briefly vortex the tube and use 5 µl per lane. There is no need to add any additional loading buffer to the vial of ready-to-load PureView™ 100 bp DNA Ladder which contains two loading and tracking dyes. Xylene cyanol FF and Orange G mimic the migration of 4,000 bp and 50 bp DNA fragments during electrophoresis.