AzuraProof™ HiFi PCR Red Mix
AzuraProof™ HiFi PCR Red Mix is an optimized 2x hot start master mix comprised of high-fidelity DNA Polymerase, optimized reaction buffer, MgCl2, ultra-pure dNTPs, and an inert red loading dye. AzuraProof™ HiFi PCR Red Mix provides greater yields with less optimization than other 2x high-fidelity master mixes, even in amplification of GC rich targets, DNA of sub-optimal purity, and long templates.
In order to confirm the ideal reagent for your application, please click here for our PCR Selection Guide.
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AzuraProof™ HiFi PCR Mix is ideal for fast cycling, as the polymerase exhibits a rapid elongation rate of 10 seconds per kb. The ready-to-use 2x mix is also highly efficient in long PCR with amplicons greater than 15 kb. With an error rate which is 75x lower than Taq Polymerase, AzuraProof™ HiFi PCR Mix is recommended for applications which require extremely high fidelity such as cloning, NGS applications, and mutagenesis.
For applications where a colorless version is preferable, we also offer AzuraProof™ HiFi PCR Mix.
Applications
Ultra-high fidelity PCR for Cloning, Crude Sample PCR, Mutagenesis, NGS Applications, Next-Generation Re-Sequencing, Protein Expression, and Long PCR > 15 kb.
Ultra High-Fidelity
AzuraProof™ HiFi PCR Red Mix exhibits fidelity that is >75x higher than Taq Polymerase and exhibits both 5'→ 3' DNA polymerase activity and 3'→ 5' proofreading exonuclease activity.
Speed and Robust Amplification
AzuraProof™ HiFi PCR Red Mix has a rapid elongation rate of 10 sec/kb, which is 4x faster than Taq. The master mix delivers robust amplification of difficult targets while maintaining sequence accuracy.
Convenience of Minimal Optimization
Available in 2x ready-to-use colorless and red master-mix format for direct gel-loading and high-throughput.