AzuraQuant™ Probe 1-Step qPCR Mix NoRox

The AzuraQuant Probe 1-Step qPCR Mix NoRox is powered by Azura HS Taq DNA Polymerase, AzuraSprint Reverse Transcriptase, and an optimized buffer chemistry, providing robust first-strand cDNA synthesis and real-time PCR in a single tube. The Mix delivers earlier quantification cycle values (Ct) and broad range detection for increased sensitivity, speed, reliability and reproducibility.

The AzuraQuant Probe 1-Step qPCR Mix NoRox can be used to quantify a specific target RNA from either total RNA or mRNA while reducing the number of pipetting steps and time to result. The AzuraQuant Probe 1-Step qPCR Mix NoRox requires little if any optimization and employs rapid antibody-mediated hot-start activation and a processive enzyme chemistry, providing unmatched performance in multiplexing.

Applications

• Multiplex RT-qPCR
• TaqMan® gene expression analysis
• Pathogen detection
• Viral Quantitation
• Biomarker discovery
• Gene knock-down validation
• Microarray validation and detection of low copy number targets

Flexibility
Compatible with standard and fast cycling instruments and a wide range of cycling parameters.

Accurate Quantification
Hot-Start chemistry reduces primer-dimer formation and allows room-temperature assembly.

Superior Multiplex Capability
Advanced formulation provides unrivalled sensitivity in singleplex as well as complex multiplex assays.