Purity of dNTPs is essential for any PCR, Real-Time PCR and cDNA Synthesis application as inhibitors and contaminants can interfere with DNA polymerases and template. PureStrand™ Ultra-Pure dNTPs are purified using exacting processes to eliminate the presence of PCR-specific inhibitors such as tetraphosphates and pyrophosphates. The presence of contaminating impurities can result in a decrease in amplification sensitivity and product yield.
The performance of each lot of PureStrand 40 mM dNTP Mix is tested in PCR, long PCR and qPCR for sensitivity and reproducibility.
For availability and pricing of bulk dNTPs, please contact us.
- >99% Triphosphate Purity by HPLC
- Free of contaminating impurities and PCR inhibitors
- DNase, RNase, Nickase and Protease Free
- Supplied as ready-to-use 40 mM Mix in ultra-pure water
- Reduces pipetting steps and risk of contamination
- Shelf-Life of 24 months at -20°C
Usage Guidelines
PureStrand 40 mM dNTP Mix is comprised of 10 mM of each dNTP. We suggest the use of 1.25 µl of 40 mM dNTP Mix in 50µl PCR volumes and AZ-1261 is sufficient for 400 PCR reactions of 50µl.