TruFi™ 2x PCR Mix
TruFi™ 2x PCR Mix is a new generation ultra high-fidelity master mix developed specifically to overcome the many disadvantages of conventional proof-reading enzymes such as poor sensitivity, significant inhibition in crude PCR assays, long extension times and the tedious optimization of buffer conditions.
Couldn't load pickup availability
TruFi™ exhibits robust 5'→ 3' DNA polymerase activity and 3'→ 5' proofreading exonuclease activity with an error rate of 4.55 x 10-7. Several point mutations have selectively modified specific amino acid residues in order to improve protein solubility and performance across a wide range of ionic conditions.
The hydrophilic nature of TruFi™ provides for significant improvements to reaction processivity for faster cycling, greater sensitivity and less inhibition with crude DNA samples. TruFi™ 2x PCR Mix is a ready-to-use format especially convenient for high-throughput applications.
Applications
• Cloning
• Crude Sample PCR
• Mutagenesis
• Next-Generation Resequencing
• Protein Expression
• Microarray
• Long PCR up to 10 kb
Ultra-High Fidelity
>50-fold higher fidelity than Taq Polymerase formulations
Robust Amplification
Provides greater yields with lower enzyme amounts than other DNA polymerases, even in crude PCR reactions with known inhibitors
Convenience of Minimal Optimization
TruFi™ 2x PCR Mix is optimized for broad compatibility with various amplicons including complex samples
Flexibility
TruFi™ is available as a ready-to use 2x PCR mix or separate components to suit a wide range of existing protocols